Photo-crosslinking UAA in live cells
(No MS involved)
|
CXCR4(GPCR)-T140
|
Protein-
small molecule
|
Protein-
small molecule
|
F189 is important for binding T140 ligand
|
(Grunbeck et al., 2011)
|
|
CCR5(GPCR)-maraviroc
|
Protein-
small molecule
|
Protein-
small molecule
|
New contact residues identified
|
(Grunbeck et al., 2012)
|
|
PGRMC1-Insig-1 |
Protein-protein |
Protein-protein |
New interfaces
identified |
(Suchanek et al., 2005) |
|
AT1R(GPCR)-arrestin |
Protein-protein |
Protein-protein |
Ligand-dependent interface change, newly found intracellular loop
engagement in the interface |
(Gagnon et al., 2019) |
|
Antibody- EGFR |
Protein-protein |
Protein-protein |
Roles of
interfacial residues in binding |
(Bridge et al., 2019) |
|
MurJ-LipidII |
Protein-lipid |
Protein-lipid |
Critical interfacial
residues responsible for transient LipidII binding |
(Rubino et al.,
2020) |
Photo-crosslinking UAA |
LptD-LptE |
Protein-protein |
Protein-protein |
Critical interfacial residues |
(Freinkman et al., 2011) |
|
DegP-substrate |
Protein-protein |
Protein-protein |
New substrates
identified by a photoactivatable UAA at the substrate recognition site |
(He et al., 2017) |
|
Histone H3-various ligands |
Protein-protein |
Protein-protein |
Weak
and transient PPIs identified |
(Yang et al., 2020) |
|
HdeA dimer |
Protein-protein |
Protein-protein |
pH-dependent changes
in the dimer interface |
(Yang et al., 2016) |
|
HdeA - DegP |
Protein-protein |
Protein-protein |
HdeA binding to
multiple interfacial regions in DegP |
|
|
Rho-RTKN |
Protein-protein |
Protein-protein |
RTKN interaction with
RohA through a similar binding interface as that on PKN |
|
Photo-crosslinker, cleavable, MS label |
MAP kinase - MKK4 peptide |
Protein-peptide |
Protein-peptide |
Unique binding interface of an
intrinsically disordered peptide within a folded protein domain |
(Parker et al., 2018) |
|
E6AP(E3) - UbcH7(E2) |
Protein-protein |
Protein-protein |
Previously
unrecognized catalytic residues of the E6AP ligase |
(Krist and Statsyuk
2015) |
Photo-crosslinker, heterobifunctional |
SurA-Unfolded OmpX |
Protein-protein |
Protein-protein |
Multi-site binding of OmpX to SurA |
(Calabrese et al., 2020) |
|
Sky-OmpA |
Protein-protein |
Protein-protein |
Unique residues and
sub-regions involved in transient interaction between Skp and denatured
OmpA |
(Horne et al., 2018) |
Photo-activatable cholesterol
|
GlyR - cholesterol
|
Protein-lipid
|
Protein-lipid
|
Unique cholesterol binding interfaces of GlyR
Cholesterol concentration-dependent changes in the interface
|
(Ferraro and Cascio 2018)
|
Lys/Asp/Glu-cross-reactive chemical linker |
Yeast proteasome |
Protein-protein |
Protein-protein |
Dynamic and disordered interface of
subunits (RPN2, RPN13) |
(Mintseris and Gygi 2020) |
|
BAX oligomer |
Protein-protein |
Protein-protein |
The unstructured N
terminus and newly exposed α1 of BAX adjacent to one another in the
oligomeric state |
(Hauseman et al., 2020) |
Heterobifunctional chemical crosslinker |
RAP1B-Glycan |
Protein-glycan |
Protein-glycan |
Different contact residues of RAP1B with glycan
variants |
(Xie et al., 2021) |
|
MDA5 filament |
Protein-protein |
Protein-protein |
Configuration of
filaments |
(Wu et al., 2013) |
Amine-reactive
chemical linker
|
p53 tetramer
|
Protein-protein
|
Protein-protein
|
DNA-dependent changes in the dimer-dimer interface
|
(Arlt et al., 2017)
|
|
p53 tetramer |
Protein-protein |
Protein-protein |
Flexible C-terminal |
(Arlt et al., 2015) |
|
NLRP1-DPP9 |
Protein-protein |
Protein-protein |
CLMS data used for
validation of the atomic model revealed by the cryo-EM |
(Hollingsworth
et al., 2021) |
|
SLAIN2, CLASP2, ch-TOG |
Protein-protein |
Protein-protein |
SLAIN2
showed crosslink patterns contradictory to previous x-ray crystal
structure |
(Liu et al., 2017) |
|
hIRE1α LD
oligomer
|
Protein-protein
|
Protein-protein
|
Critical residues in the oligomeric interface
|
(Karagöz et al., 2017)
|
Structure refinement
from published dataset
|
Rpn2-Rpn13
|
Protein-protein
|
Protein-protein
|
The intrinsically disordered C-terminal interface of Rpn2
|
(Gong et al., 2020)
|