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Multi-omics analysis reveals a link between Brassica -specific miR1885 and rapeseed tolerance to low temperature
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  • Pengfei Xu,
  • Wenting Zhang,
  • Xuan Wang,
  • Yantao Zhu,
  • Wanqi Liang,
  • Yuke He,
  • Xiang Yu
Pengfei Xu
Shanghai Jiao Tong University School of Life Sciences and Biotechnology

Corresponding Author:xupengfei2022@sjtu.edu.cn

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Wenting Zhang
Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences
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Xuan Wang
Shanghai Normal University
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Yantao Zhu
Hybrid Rapeseed Research Center of Shaanxi Province
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Wanqi Liang
Shanghai Jiao Tong University School of Life Sciences and Biotechnology
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Yuke He
Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences
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Xiang Yu
Shanghai Jiao Tong University School of Life Sciences and Biotechnology
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Abstract

Brassica crops include various edible vegetable and plant oil crops, and their production is limited by low temperature. The key regulators of low-temperature resistance in Brassica remain largely unexplored. To identify post-transcriptional regulators of plant response to low temperature, we performed small RNA profiling, and found that 16 known miRNAs responded to cold treatment in Brassica rapa. The cold response of seven of those miRNAs were further confirmed by qRT-PCR and/or northern blotting analyses. In parallel, a genome-wide association study of 220 accessions of Brassica napus identified four candidate MIRNA genes, all of which were cold-responsive, at the loci associated with low temperature resistance. Specifically, these large-scale data analyses revealed a link between miR1885 and the plant response to low temperature in both B. rapa and B. napus. Using 5′ rapid amplification of cDNA ends approach, we validated that miR1885 can cleave its putative target gene transcripts, Bn.TIR.A09 and Bn.TNL.A03, in B. napus. Furthermore, overexpression of miR1885 in B. napus decreased the mRNA abundance of Bn.TIR.A09 and Bn.TNL.A03 and resulted in increased sensitivity to low temperature. Knocking down of miR1885 in B. napus led to increased mRNA abundance of its targets and improved rapeseed tolerance to low temperature. Together, our results suggested that the loci of miR1885 and its targets could be potential candidates for the molecular breeding of low temperature-tolerant Brassica crops.