The prognosis of patients with ovarian cancer (OC) is highly heterogeneous which hinders to make an appropriate clinical decision. The study aimed to stratify patients’ prognoses by establishing a risk assessment model in the context of mRNA levels of immune-related genes (IRGs). Comprehensive bioinformatics analyses were done using datasets from The UCSC Xena platform, ICGC Data Portal, The Cancer Genome Atlas (TCGA), and the Genotype‐Tissue Expression (GTEx) project. LASSO regression was done to determine the independence of associations of specific factors with overall survival (OS). Nomogram that combined the independent prognostic factors was constructed to predict the OS of OC patients. The tumor microenvironment and immune response were estimated by cell type identification via estimating the relative subset of known RNA transcripts (CIBERSORT) and immunophenoscore (IPS). Overall ten IRGs were significantly associated with the OS probability and were used for the prognostic model construction of OC patients. According to the prognostic model, ovarian cancer samples were identified as high- or low-risk group. A nomogram containing risk score, stage, histological grade and age could significantly predict 1-year, 3-years, and 5-years OS probability of OC patients, as well as a higher IPS score and a higher immunoreactivity phenotype, which were probably correlated with better immunotherapy response and good prognosis. In conclusion, we established a reliable IRGs-based risk model with potential prognostic value for patients with ovarian cancer. Further studies are needed to confirm these prognosis-associated biomarkers.

Objective: To evaluate the long-term outcomes of vulvar or vaginal cancer patients undergoing laparoendoscopic single-site inguinal lymphadenectomy (LESS-IL). Design: Prospective cohort study. Setting: Single centre in Zhongda Hospital, Southeast University from July 2018 to April 2021. Population: A total of 16 women diagnosed with vulvar or vaginal cancer. Methods: Women with vulvar or vaginal cancer underwent LESS-IL performed by the same surgeon from July 2018 to April 2021. Main outcome measures: Short- and long-term complications, and oncologic outcomes Results: A total of 16 patients undergoing 28 LESS-IL procedures were identified, among whom 4 underwent unilateral LESS-IL. Median numbers of excised groin lymph nodes were 9.0 (6.5-11.8) and 10.5 (8.3-12.0) in each left and right groin, respectively. Short-term complications occurred in 4 (25%) patients, including 18.7% lymphocele and 6.3% wound infection. Long-term complications regarding lower limb lymphedema appeared in 6 (37.5%) patients. Most of the short- and long-term complications were Clavien-Dindo 1 or 2, accounting for 90% of all postoperative issues. After a median follow-up of 23 (17.3‐31.8) months, only 1 patient (6.3%) had isolated inguinal recurrence at 13 months postoperatively. No local or distant recurrence occurred. Conclusion: Our results suggest that LESS-IL is associated with little incidence of complications and promising oncologic outcomes, further demonstrating the safety and feasibility of LESS-IL technique in patients requiring IL. Keywords Laparoendoscopic single-site inguinal lymphadenectomy, vulvar cancer, vaginal cancer, compliaction, oncologic outcome