Toxicity Assay of Repeated Administration and Skin Irritation
Test
New Zealand rabbits (both sexes, 14-16 weeks, 2.3-2.7 kg) were purchased
from Qindao Kangda Biological Technology (Shandong, China), randomly
assigned into four groups (n=10) contained control-group (blank
solvent), allicin low-dose group (2 mg/g, measured with alliin), allicin
middle-dose group (4 mg/g, measured with alliin), and allicin high-dose
group (8 mg/g, measured with alliin). After four consecutive months of
topical administration of allicin, body weight, organ weight,
hematologic and clinical chemistry analysis were evaluated. Skin
irritation included erythema and edema and was evaluated based on the
above criteria. Stimulus Scores = Total Cumulative Stimulus Scores /
Total Observation Times. Stimulation grade: without irritation (0-0.49),
mild irritation (0.5-2.99), moderate irritation (3.0-5.99), severe
irritation (6.0-8.00).