Polyploids are cells or organisms with a genome consisting of more than two sets of homologous chromosomes. Polyploid plants have important traits that facilitate speciation and are thus often model systems for evolutionary, molecular ecology and agricultural studies. However, due to their unusual mode of inheritance and dou-ble-reduction, diploid models of population genetic analysis cannot properly be ap-plied to polyploids. To overcome this problem, we developed a software package en-titled VCFPOP to perform a variety of population genetic analyses for autopolyploids, such as parentage analysis, analysis of molecular variance, principal coordinates analysis, hierarchical clustering analysis and Bayesian clustering. We make this soft-ware freely available, downloadable from http://github.com/huangkang1987/vcfpop.