Results
Immune responses to suspected drugs were detected in both PBMC and BFC
samples of two donors (Case 1 in response to ceftriaxone and Case 4 to
oxypurinol), with BFC eliciting stronger responses. For two other
donors, only BFC samples showed a response to meloxicam(Case 2) or
sulfamethoxazole and its 4-Nitro metabolite (Case 3). Consistently, flow
cytometry revealed a greater proportion of IFN-γ-secreting cells in the
BFC compared to PBMC. BFC cells from Case 3 were also enriched for
memory/activation/tissue-recruitment markers over PBMC.