Objectives: In this study, it was aimed to evaluate the effect of beta-blocker use on left ventricular global longitudinal strain (LV Gls) in echocardiography in patients with MB (Myocardial Bridge). Patients and methods: A single-center study was conducted prospectively in which patients with coronary angiography were performed and myocardial bridging was detected between January 2019 and February 2022. A total of 100 patients (38 females, 62 males; mean age: 57.3±9.6 years; range, 32-75 years) with myocardial bridging were recruited consecutively and strain echocardiography was performed. Results: All patients in our study were followed in sinus rhythm, and there was a statistically significant difference in mean heart rate between groups I and II (p<0,001). LV Gls was found to be statistically significant in favor of group II in the comparison of group I [(-12,57)±3 vs. (-15,92)±2,9] and group II (p<0.001). Conclusion: LV Gls is positively affected in echocardiography thanks to the negative chronotropic effect obtained with beta-blocker therapy in patients with MB detected in coronary angiography.