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Identification of vacuolar phosphate influx transporters in Brassica napus
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  • Bei Han,
  • Chuang Wang,
  • Tao Wu,
  • Junjun Yan,
  • Aosheng Jiang,
  • Yu Luo,
  • Hongmei Cai,
  • Guangda Ding,
  • Philip White,
  • Yu Liu,
  • Xu Dong,
  • Fangsen XU,
  • Sheliang Wang,
  • Lei Shi
Bei Han
National Key Laboratory of Crop Genetic Improvement

Corresponding Author:hanbei.hzau.edu.cn@webmail.hzau.edu.cn

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Chuang Wang
Huazhong Agricultural University College of Resources and Environment
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Tao Wu
National Key Laboratory of Crop Genetic Improvement
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Junjun Yan
National Key Laboratory of Crop Genetic Improvement
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Aosheng Jiang
National Key Laboratory of Crop Genetic Improvement
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Yu Luo
National Key Laboratory of Crop Genetic Improvement
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Hongmei Cai
Huazhong Agricultural University College of Resources and Environment
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Guangda Ding
National Key Laboratory of Crop Genetic Improvement
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Philip White
National Key Laboratory of Crop Genetic Improvement
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Yu Liu
Zhejiang University College of Life Sciences
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Xu Dong
Chinese Academy of Sciences State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics
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Fangsen XU
National Key Laboratory of Crop Genetic Improvement
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Sheliang Wang
National Key Laboratory of Crop Genetic Improvement
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Lei Shi
National Key Laboratory of Crop Genetic Improvement
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Abstract

Recent progress has shown that vacuolar Pi transporters (VPTs) are important for cellular Pi homeostasis against external Pi variations in Arabidopsis and rice, while it is poorly understood for the identity and regulatory mechanism of VPTs in Brassica napus ( B. napus). Here, we identified two vacuolar Pi influx transporters BnA09PHT5;1b and BnCnPHT5;1b in B. napus and uncovered their necessity for cellular Pi homeostasis through functional analysis. BnPHT5;1bs are the homologs of Arabidopsis AtPHT5;1 with the similar sequence, structure, tonoplast localization, and VPT activity. BnPHT5;1b double mutants had smaller shoot growth and higher shoot cellular Pi than the wild-type B. napus, which are largely different from the report in At PHT5;1 mutant, suggesting PHT5;1-VPTs play a distinct mechanism of cellular Pi homeostasis in seedlings of B. napus and Arabidopsis. By contrast, disruption of BnPHT5;1b genes slowed vegetative growth accompanied by Pi toxicity in floral organs, reduced seed yield and impacted seed traits, agreeing with the role of AtPHT5;1 in floral Pi homeostasis. Taken together, our studies identified two vacuolar Pi influx transporters in B. napus and revealed the distinct and conserved regulatory mechanisms of BnPHT5;1bs in cellular Pi homeostasis in this plant species.
15 Apr 2022Submitted to Plant, Cell & Environment
20 Apr 2022Submission Checks Completed
20 Apr 2022Assigned to Editor
26 Apr 2022Reviewer(s) Assigned
05 Jun 2022Review(s) Completed, Editorial Evaluation Pending
14 Jun 2022Editorial Decision: Revise Minor
19 Jul 20221st Revision Received
19 Jul 2022Submission Checks Completed
19 Jul 2022Assigned to Editor
27 Jul 2022Reviewer(s) Assigned
14 Aug 2022Review(s) Completed, Editorial Evaluation Pending
15 Aug 2022Editorial Decision: Revise Minor
18 Aug 20222nd Revision Received
18 Aug 2022Submission Checks Completed
18 Aug 2022Assigned to Editor
18 Aug 2022Review(s) Completed, Editorial Evaluation Pending
18 Aug 2022Editorial Decision: Accept
Sep 2022Published in Plant, Cell & Environment. 10.1111/pce.14423