2.1 Sample collection
A total of 92 adults of C. capitata were collected in infected
orchards from six sites across all biogeographic regions where the
species occurs (Afrotropical, Palaearctic, Neotropical, and
Australasian) between 2005 and 2012 (Figure 1; Table 1). All flies were
preserved in 80% ethanol and kept at -20ºC until used for DNA
extraction.