Comparative analysis of magnetically activated cell sorting and
ultracentrifugation for exosome isolation from human mesenchymal stem
cell culture supernatant
- Eda Ciftci-Dede,
- Naz Bozbeyoğlu,
- İhsan Gürsel,
- Feza Korkusuz,
- Feray Bakan-Misirlioglu,
- PETEK KORKUSUZ
Eda Ciftci-Dede
Hacettepe Universitesi
Corresponding Author:eda.ciftci@hacettepe.edu.tr
Author ProfileAbstract
Exosomes derived from mesenchymal stem cells regulate cell migration,
proliferation, differentiation, and synthesis of the extracellular
matrix. The ultracentrifugation method is the gold standard for exosome
isolation with its simple protocol, high exosomal yield, allowing to
work with high volume of sample features, but low purity and requirement
of specialized equipment. Optimal isolation does not exist yet for
obtaining the exosomes derived from mesenchymal stem cells for clinical
usage. We hypothesized that magnetic-activated cell sorting may provide,
effective and rapid exosomes derived from human mesenchymal stem cells
when compared to ultracentrifugation. We, therefore, aimed to compare
the efficiency of magnetic-activated cell sorting and
ultracentrifugation for human bone marrow mesenchymal stem cell-derived
exosome isolation from culture media by protein quantification, surface
biomarker analysis, particle size and number analysis, and morphological
analysis in vitro. Magnetically activated cell sorting provided a higher
purity and amount of exosome when compared to ultracentrifugation.
Magnetically activated cell sorting isolated samples exhibited the
presence of magnetic beads morphologically. The particle number of the
magnetic-activated cell sorting group was higher than the
ultracentrifugation. In conclusion, the isolation of exosomes derived
from mesenchymal stem cells by magnetic-activated cell sorting presents
a quick and reliable method to collect them at high purity for clinical
usage.