: In this study electrospinning method was used to prepare nanofiber of chitosan polymer and polyethylene oxide (PEO) solution. Also marshmallow extract as a useful medicinal plant was loaded to it in different concentrations .Optimal electrospinning conditions included a feed rate of 0.5 ml / h, a voltage of 10 kV, a needle-to-collector distance of 200 mm and a collector rzzzotation speed of RPM200. SEM images of nanofibers prepared with marshmallow extract loaded show a network structure of non-woven fibers with no spheres and the difference between the mean diameters of their fibers was not significantly different (P = 0.315). Viscosity measurements show that the lowest viscosity is related to 2.5% chitosan solution and 10% marshmallow extract, which is significantly less than chitosan solution alone (P <0.0001). The highest electrical conductivity is related to Chitosan solution of marshmallow extract is 20% and PEO is 2.5%. On the other hand, increasing the concentration of the extract in the nanofibers increases the surface tension and the tensile test shows that the difference in the mean mechanical tension between the study groups was significantly different (P = 0.011) .The FTIR study of the prepared nanofibers shows no reaction Between marshmallow extract and chitosan and polyethylene oxide, which leads to the production of new compounds, did not occur. It was a mixture of biomaterials. Drug release was also performed by calculating the area under the curve.