Sample collection, library construction and sequencing
A mature female striped catfish, cultivated in Wenchang City, Hainan
Province, China, was selected for whole genome sequencing. Genomic DNAs
were extracted from pooled muscle tissues using a genomic DNA isolation
kit (Qiagen, Hilden, Germany). For
the routine genome shotgun
sequencing, a 500-bp insert library was constructed, followed by
paired-end sequencing
on
an Illumina Hiseq Xten platform (Illumina Inc., San Diego, CA, USA). For
the long-read sequencing, a Nanopore library with an insert-size of 20
kb was prepared using the extracted genomic DNAs, and subsequently
sequenced on a Nanopore platform (Nanopore Technologies, Oxford, Head
Office, UK). The Hi-C library, constructed with the same DNA pool, was
sequenced on the same Illumina Hiseq Xten platform.
In order to assist genome
annotation, total RNAs were
extracted from various tissues, including muscle, gill, skin, liver and
kidney. Individual cDNA library was constructed for transcriptome
sequencing (RNA-seq) on the same
Illumina Hiseq Xten platform.
This project was reviewed and approved by the Institutional Review Board
on Bioethics and Biosafety of BGI, China (No. FT 18134).