Genome assembly
Before assembly, LoRDEC (Salmela & Rivals, 2014) was applied for calibration of the long sequencing reads along with the clean short reads. Subsequently, minimap2 (Li, 2018) was employed to assemble the corrected long reads to contigs. Genome polishing was preformed two rounds with different strategies. In brief, for the first round, the contigs were polished via Racon v1.3.1 (Vaser et al., 2017) based on uncorrected Nanopore long reads; for the second round, the clean short reads were used to polish the contigs with pilon (Walker et al., 2014). After heterozygosity reducing with Redundans (Pryszcz & Gabaldón, 2016), we finally obtained a draft genome for the striped catfish.