Sample collection, library construction and sequencing
A mature female striped catfish, cultivated in Wenchang City, Hainan Province, China, was selected for whole genome sequencing. Genomic DNAs were extracted from pooled muscle tissues using a genomic DNA isolation kit (Qiagen, Hilden, Germany). For the routine genome shotgun sequencing, a 500-bp insert library was constructed, followed by paired-end sequencing on an Illumina Hiseq Xten platform (Illumina Inc., San Diego, CA, USA). For the long-read sequencing, a Nanopore library with an insert-size of 20 kb was prepared using the extracted genomic DNAs, and subsequently sequenced on a Nanopore platform (Nanopore Technologies, Oxford, Head Office, UK). The Hi-C library, constructed with the same DNA pool, was sequenced on the same Illumina Hiseq Xten platform.
In order to assist genome annotation, total RNAs were extracted from various tissues, including muscle, gill, skin, liver and kidney. Individual cDNA library was constructed for transcriptome sequencing (RNA-seq) on the same Illumina Hiseq Xten platform.
This project was reviewed and approved by the Institutional Review Board on Bioethics and Biosafety of BGI, China (No. FT 18134).