Genome size estimation based on the routine k-mer method
Short reads generated from the whole
genome shotgun sequencing were filtered using SOAPnuke v1.0 (Chen et
al., 2018). Clean short reads were then used for genome size estimation
based on a 17-mer distribution according to the following formula:
Genome Size=Kmer_num/Kmer_depth, where kmer_num is the total number
of k-mers and Kmer_depth represents the sequencing depth of each unique
k-mer with the highest frequency.