Results

Method comparison

HEV IgG

The Mikrogen recom Well HEV IgG and the LIAISON Murex anti-HEV IgG immunoassays were compared by analyzing 113 blood samples (either IgM, IgG positive/borderline or IgG and IgM positive/borderline in the Mikrogen test) and 49 IgG and IgM negative samples. Table 1 shows the 100% qualitative agreement between the results of both assays. The sensitivity and the specificity is 100 % [95% CI, 0.96 to 1.00] and 100 % [95% CI, 0.91 to 1.00], respectively. In addition, Table 1 demonstrates the qualitative agreement including the immunoblot results. Again, the sensitivity is 100 % [95% CI, 0.96 to 1.00]. No specificity could be determined as no negative samples confirmed by immunoblot were included.
Table 1 – Qualitative agreement of the recomWell/recomLine HEV IgG and the LIAISON® Murex anti-HEV IgG immunoassays. The raw data can be found in the supplement. In the recomWell HEV IgG test, a borderline result means that the antibody concentration is in the range of 20 to 24 IU/ml.