Patterns of genetic differentiation.
To estimate the extent of genetic differentiation across the different stages of the linear transport infrastructure project, we estimated F and G statistics (Jost 2008; Jost et al. 2018) and the mutual information I (Sherwin et al. 2006; Sherwin 2010; Sherwinet al. 2017) using GenAlex program V6. (Peakall & Smouse 2006; Peakall & Smouse 2012). Mutual information is a measure of genetic differentiation that not only avoids undue emphasis on rare or common alleles (unlike allele-sharing measures or Fst/Gst/Dest respectively), but also is purely a measure of differentiation, without influence of either within-location variation or total variation over all locations (unlike Fst/Gst/Dest) (Sherwinet al. 2017, 2021).