Statistical experimental design and statistical analyses
A full factorial design at three levels (Modde v12.0 software, Umetrics, Sweden) was used to investigate the effects of studied factors on the yield and secretion of the recombinant protein. Studied factors were KNO3, NAA and MeJA.
Statistical analyses were conducted with IBM SPSS Statistics 25 software. Normality of the data was assessed with Shapiro-Wilk’s test. Unless otherwise described, one-way ANOVA was used together with Tukey HSD as post-hoc test when homogeneity of variances allowed (Levene test p>0.05). With data comprising unequal variances, Dunnett T3 test was used. Confidence level p<0.01 was used throughout the data.