Factor 1st secretion induction 2nd secretion induction 3rd secretion induction
KNO 5-15 g/l 2-26 g/l 15-35 g/l
NAA 1-20 mg/l - -
MeJA 50-250 µM 9-91 µM 10-50 µM
DoE Full Factorial design in two levels Central Composite Orthogonal (CCO) design with star points Central Composite Orthogonal (CCO) design with star points
Number of experimental (and central) points 11 (3) 11 (3) 11 (3)