2.2 Sequence analysis
The protein sequence of Human Iroquois family members like IRX1
(P78414), IRX2 (Q9BZI1), IRX3 (P78415), IRX4 (P78413), IRX5 (P78411) and
IRX6 (P78412) were retrieved from UniProt (accession numbers in
brackets). IRX4 (P78413) is a 519 amino acid long protein with DNA
binding region, in positions 143-204. The homeodomain consists of 62
amino acids in the IRX4 protein. Multiple sequence alignment and
phylogenetic tree analysis of IRX family members was performed using
Clustal Omega 39. The
62 amino acid homeodomain sequence was checked for mutations in the
region using the Catalogue of Somatic Mutations in Cancer (COSMIC)
database 40.