2.9 Cell-penetrating peptide identification
The homeodomain sequence of IRX4 was subjected to the CPPsite 2.0, a
database of cell-penetrating peptides60. CPP site 2.0
contains experimentally validated cell-penetrating peptides and this
server was used to determine the efficacy of IRX4 sequence to be
considered in that category. SkipCPP-Pred was used to predict the
sequence having the highest cell-penetrating potential61. SkipCPP-Pred
(http://server.malab.cn/SkipCPP-Pred/Index.html) is a
sequence-based computational predictor of cell-penetrating peptides that
uses an algorithm k-skip n-gram combined with a random forest
classifier.