2.6 | Putative identification of the significant metabolites
Metabolite features that are unique to each species or are shared among some/all of them were identified using a Venn Diagram (http://bioinformatics.psb.ugent.be/webtools/Venn/). To obtain putative prediction of the metabolic pathways that the identified metabolite features are assigned to, we compared the accurate masses of these features against the annotated metabolite database ofArabidopsis thaliana using the “Functional Analysis” module and the Gene Set Enrichment Assay (GSEA) tool of MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/MetaboAnalyst/ModuleView.xhtml). Using these predictions, we compared the metabolic pathways that are shared by some/all of the species or are unique to each.