2.6 | Putative identification of the significant
metabolites
Metabolite features that are unique to each species or are shared among
some/all of them were identified using a Venn Diagram
(http://bioinformatics.psb.ugent.be/webtools/Venn/). To obtain
putative prediction of the metabolic pathways that the identified
metabolite features are assigned to, we compared the accurate masses of
these features against the annotated metabolite database ofArabidopsis thaliana using the “Functional Analysis” module and
the Gene Set Enrichment Assay (GSEA) tool of MetaboAnalyst 5.0
(https://www.metaboanalyst.ca/MetaboAnalyst/ModuleView.xhtml).
Using these predictions, we compared the metabolic pathways that are
shared by some/all of the species or are unique to each.