THE USE OF IONTOPHORESIS WITH HYALURONIC ACID ASSOCIATED WITH GOLD
NANOPARTICLES ACCELERATES WOUND HEALING IN RATS
Abstract
This study aimed to investigate the effects of iontophoresis with HA
associated to GNPs solution in an epithelial lesion model. Fifty Wistar
rats (n=10/group) were randomly assigned to the following groups:
epithelial lesion (EL); (EL+MIC); (EL+MIC+HA); (EL+MIC+GNPs);
(EL+MIC+HA-GNPs). The animals induced to an epithelial lesion and
treatment started 24 hours after injury with microcurrents (300µA)
containing gel with HA (0.9%) and/or GNPs (30mg/Kg) in the electrodes
(1mL) for seven days. The animals were sacrificed 12 hours after the
last treatment application. Results demonstrated a reduction in the
levels of pro-inflammatory cytokines (IFNϒ, IL-1β, TNFα, IL6) in the
group in which the therapies were combined; an increase in the levels of
anti-inflammatory cytokines (IL-4, IL-10) and growth factors (FGF, TGFβ)
in EL+MIC+HA and EL+MIC+HA-GNPs groups. As for dichlorofluorescein (DCF)
and nitrite levels, decreased in the combined therapy group when
compared to the control group, as well as the oxidative damage (carbonyl
and sulfhydryl). In antioxidant defense, there was an increase in
glutathione (GSH) and a decrease in superoxide dismutase (SOD) in the
combination therapy group. Histological analysis showed a reduction in
the inflammatory infiltrate in groups treated with MIC and in the
combination therapy group. An increase in contraction of the wound area
was obtained in all treated groups when compared to the control group,
proving that the proposed therapies are effective in the process of
epithelial healing. The results of this study demonstrated that the
associated therapies favor the tissue repair process more significantly
compared to the isolated therapies.