Peroxide Value (PV)
In brief, the oil treatments (0.001-0.3 g) were diluted in chloroform-methanol (9.8 mL, 7:3 v/v). Then, 50 µL of the clear solution of iron (II) chloride, and 50 µL ammonium thiocyanate solution (30% w/v) were added on a vortex mixer for 5 s. The solution was saved for 5 min at 25 °C, and then the absorbance of the solution was measured at 500 nm (Shantha and Decker, 1994).