Β-Carotene Bleaching Method
Briefly, 0.2 mg of the β-carotene was dissolved in 10 mL of chloroform to prepare t he β-carotene solution. Then, 20 mg linoleic acid and 200 mg Tween 40 were added to 1 mL of β-carotene solution. After evaporation of chloroform, 50 mL of oxygenated distilled water was added to the mixture by vigorous shaking. Finally, 100 µL of antioxidant compounds was added to 4 mL of the prepared emulsion. The emulsion absorbance was recorded at 470 nm immediately at t = 0 min. The emulsion vial was stored in a water bath at 45 °C for 35 min, and finally, the emulsion absorbance was recorded (Shahsavari et al., 2008). The percentage inhibition (%I) was calculated from Eq. (2):
\(\%I=\frac{A_{S(t)}-\ A_{C(t)}}{A_{C(0)}-\ A_{C(t)}}\ \times 100\)(2)
where AC(t) and AS(t) are the absorbance of the control and sample at t =30 min, respectively, and AC(0) is the control absorbance at t = 0 min.