2.2 Genome size assessment

The clean reads (152 Gb) from the 350 bp insert-size Illumina library were used to estimate the genome size based on the 17-mer method. Jellyfish (Marçais & Kingsford, 2011) was used to generate the k -mer frequency distribution. Genomescope (Ranallo-Benavidez, Jaron, & Schatz, 2020) was employed to estimate the genome size. The k -mer number was 33,414,462,943, with a peak depth of 34. The estimated genome size of S. tetraptera was ~ 982.78 Mb (Figure S1, Table S1).