Figure 1| Library preparation and high-throughput evaluation of
Cpf1 guide RNA activity.
(a) Representation of an oligonucleotide containing pairs of target and
guide RNA sequence.
(b) A schematic of the high-throughput evaluation of Cpf1 guide RNA
activity.
(c) The distribution of guide RNA activity in screening expriment1 and
screening expriment2
(d) The pooled screening results were confirmed by cloning 11 guide RNAs
(9 of high activity and 2 of low activity) individually and measured via
transformation assay.