In this study, the Franz diffusion kinetic model and Bacitracin’s (BAC) cumulative release kinetic mathematical model are designed. The Franz diffusion mathematical release ki- netic model of Zataria Multiflora (ZM)/BAC nanofiber (NF) structure has been revealed as Zero Order, since there is no antibacterial protection effect for shelf-life, it has been analyzed over the Python program and its shelf-life has been determined as 125 days.