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Andy Vierstraete
Andy Vierstraete

Public Documents 1
Amplicon_sorter: a tool for reference-free amplicon sorting based on sequence similar...
Andy Vierstraete
Bart Braeckman

Andy Vierstraete

and 1 more

October 08, 2021
Oxford Nanopore Technologies (ONT) is a third-generation sequencing technology that is gaining popularity in ecological research for its portable and low-cost sequencing possibilities. Although the technology is primarily developed for long-read sequencing, it can also be applied to sequence amplicons. The downside of ONT is the low quality of the raw reads. Hence, generating a high-quality consensus sequence is still a challenge. We present Amplicon_sorter, a tool for reference-free sorting of ONT sequenced amplicons based on their similarity in sequence and length and for building solid consensus sequences.

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