2.2 DNA extraction
Five ml of whole blood sample was collected from the proband (III-3) and
the other family members (Ⅰ-2,
Ⅱ-1, Ⅱ-2, Ⅱ-3, Ⅱ-4, Ⅱ-5, Ⅱ-6,
Ⅲ-1 and Ⅲ-2) individually using
EDTA-anticoagulant vacuum blood collection tubes. Genomic DNA was
extracted from peripheral blood leukocytes of the collected blood
samples using a DNA extraction kit (TianGen, Beijing, China) according
to the manufacturer’s instructions. And the DNA extracts were stored at
−20℃ for analysis later.