Statistical Analysis
Flow cytometry, mass cytometry and plasma cytokine data analysis were
done using non-parametric methods in R and GraphPad Prism (Dotmatics).
Correlation of two continuous variables was performed using Spearman
Rank correlation while comparison of continuous variables against binary
categorical variables was done using Mann-Whitney U tests. PhenoGraph
and UMAP analysis were done in R using the
Cytofkit214[18]. P
values less than 0.05 were deemed to be statistically significant.
PhenoGraph and cluster analysis was performed using Cytofkit2
[18] followed by statistical analysis
using Graphpad Prism (Dotmatics).