Statistical Analysis
Flow cytometry, mass cytometry and plasma cytokine data analysis were done using non-parametric methods in R and GraphPad Prism (Dotmatics). Correlation of two continuous variables was performed using Spearman Rank correlation while comparison of continuous variables against binary categorical variables was done using Mann-Whitney U tests. PhenoGraph and UMAP analysis were done in R using the Cytofkit214[18]. P values less than 0.05 were deemed to be statistically significant.
PhenoGraph and cluster analysis was performed using Cytofkit2 [18] followed by statistical analysis using Graphpad Prism (Dotmatics).