Singapore children cohort
The active disease cohort is comprised of mixed-ethnicity Singaporean
subjects below the age of 21 years old. Similarly, clinical phenotypic
information and demographic parameters were obtained from volunteers
using a standardised survey questionnaire based on ARIA and ISSAC
guidelines. All volunteers underwent a SPT using common HDM allergensDermatophagoides pteronyssinus (DP) and Blomia tropicalis(BT) to ascertain atopy status. Fresh whole blood samples were collected
from the volunteers into Tempus™️ Blood RNA tubes (Life Technologies,
Carlsbad, Calif) for RNA isolation.