Singapore children cohort
The active disease cohort is comprised of mixed-ethnicity Singaporean subjects below the age of 21 years old. Similarly, clinical phenotypic information and demographic parameters were obtained from volunteers using a standardised survey questionnaire based on ARIA and ISSAC guidelines. All volunteers underwent a SPT using common HDM allergensDermatophagoides pteronyssinus (DP) and Blomia tropicalis(BT) to ascertain atopy status. Fresh whole blood samples were collected from the volunteers into Tempus™️ Blood RNA tubes (Life Technologies, Carlsbad, Calif) for RNA isolation.