Tslpr-/- mice show decreased
neuroinflammation in EAE model
MOG35-55 was used to induce EAE mice model (Fig. 1A). To
investigate the role of TSLPR in CNS autoimmunity, the clinical score of
EAE in Tslpr -/- andTslpr +/+ mice was determined. After EAE
induction, Tslpr +/+ mice developed into typical
monophasic EAE symptoms manifested with ascending paralysis 10-12 days
after MOG35-55imunization (Fig. 1B). By contrast,Tslpr -/- mice show delayed onset of paralysis
at 11-13 days and alleviated symptoms when compared to that inTslpr +/+ mice. Even though, EAE score of both
group peaked at day 15 (Fig. 1B). In addition, immunohistochemistry
analysis of brain tissues from Tslpr +/+ andTslpr-/- mice displayed that at day 15 after EAE
induction revealed significant reductions in the number of
CD4+lymphocytes present as inflammatory infiltrates of
the brain tissue of Tslpr-/- mice when compared
to that in Tslpr +/+ mice (Fig. 1C).
Importantly, results from western blot revealed that mice MOG35-55
injection led to reduced expression of myelin basic protein (MBP) inTslpr +/+ mice when compared to that without
MOG35-55 injection. However,Tslpr-/- mice of EAE showed restored expression
of MBP when compared to that in Tslpr +/+ mice
(Fig. 1D).