Tslpr-/- mice show decreased neuroinflammation in EAE model
MOG35-55 was used to induce EAE mice model (Fig. 1A). To investigate the role of TSLPR in CNS autoimmunity, the clinical score of EAE in Tslpr -/- andTslpr +/+ mice was determined. After EAE induction, Tslpr +/+ mice developed into typical monophasic EAE symptoms manifested with ascending paralysis 10-12 days after MOG35-55imunization (Fig. 1B). By contrast,Tslpr -/- mice show delayed onset of paralysis at 11-13 days and alleviated symptoms when compared to that inTslpr +/+ mice. Even though, EAE score of both group peaked at day 15 (Fig. 1B). In addition, immunohistochemistry analysis of brain tissues from Tslpr +/+ andTslpr-/- mice displayed that at day 15 after EAE induction revealed significant reductions in the number of CD4+lymphocytes present as inflammatory infiltrates of the brain tissue of Tslpr-/- mice when compared to that in Tslpr +/+ mice (Fig. 1C). Importantly, results from western blot revealed that mice MOG35-55 injection led to reduced expression of myelin basic protein (MBP) inTslpr +/+ mice when compared to that without MOG35-55 injection. However,Tslpr-/- mice of EAE showed restored expression of MBP when compared to that in Tslpr +/+ mice (Fig. 1D).