NLRP3 inhibition alleviates neuroinflammation
To evaluate the role of NLRP3 in the neuroinflammation of EAE, MCC950, a
potent, selective, small-molecule inhibitor of NLRP3, was used to block
canonical and noncanonical NLRP3 activation 30.
Treatment of MCC950 by i.p administration in EAE mice resulted in
significant reduction in inflammatory cells infiltration by HE staining
(Fig 4A), CD4+ cells infiltration (Fig 4B), and
restoration of myelin sheath expression by LFB staining (Fig 4C) and MBP
by western blot (Fig 4D). NLRP3 inhibition also reduced IL-1β level (Fig
4E) and TSLP level (Fig 4F) in the brain of EAE mice when compared to
that without NLRP3 inhibition.