CNS inflammation
For determination of inflammation infiltrates, brains were harvested, fixed in 4% formalin stored at room temperature. Tissue histology, including HE, NLRP3 staining, CD4 staining, and Luxol Fast Blue (LFB) staining (Servicebio, China) of brain tissue sections was performed to determine the infiltrates of inflammatory cells, CD4+T cells and myelin sheath expression.