Increased numbers of Th22 and Th17 cells in KD patients.
We first characterized the levels of different subset of effector
CD3+CD4+T cells in KD patients by
flow cytometry analysis. As shown in Fig 1, KD patients with CAL had
significantly increased numbers of
CD3+CD4+IL-22+Th22
and
CD3+CD4+IL-17+Th17
cells compared to KD patients with NCAL and HCs [Th22 (1.62 ±
0.29)×104 versus (1.10 ± 0.27%)×104versus (0.85 ± 0.21%)×104, P<0.05, Th17
(2.80 ± 0.78%)×104 versus (2.07 ±
0.66%)×104 versus (2.07 ±
0.41%)×104, P<0.05]. Furthermore, KD
patients with NCAL had significantly increased numbers of
CD3+CD4+IL-22+Th22
and
CD3+CD4+IL-17+Th17
cells compared to HCs (P<0.05) . However, we did not find a
significant difference in the numbers of
CD3+CD4+IFN-γ+Th1
cells among the different groups of patients and HCs [ (24.80 ±
4.15)×104 versus (22.22 ± 5.12)×104versus (25.33 ± 4.84)×104, P>0.05].