Increased numbers of Th22 and Th17 cells in KD patients.
We first characterized the levels of different subset of effector CD3+CD4+T cells in KD patients by flow cytometry analysis. As shown in Fig 1, KD patients with CAL had significantly increased numbers of CD3+CD4+IL-22+Th22 and CD3+CD4+IL-17+Th17 cells compared to KD patients with NCAL and HCs [Th22 (1.62 ± 0.29)×104 versus (1.10 ± 0.27%)×104versus (0.85 ± 0.21%)×104, P<0.05, Th17 (2.80 ± 0.78%)×104 versus (2.07 ± 0.66%)×104 versus (2.07 ± 0.41%)×104, P<0.05]. Furthermore, KD patients with NCAL had significantly increased numbers of CD3+CD4+IL-22+Th22 and CD3+CD4+IL-17+Th17 cells compared to HCs (P<0.05) . However, we did not find a significant difference in the numbers of CD3+CD4+IFN-γ+Th1 cells among the different groups of patients and HCs [ (24.80 ± 4.15)×104 versus (22.22 ± 5.12)×104versus (25.33 ± 4.84)×104, P>0.05].