3.1∣In vivo translation of For t 2 proteins in skin and spleen after epicutaneous vaccination
To detect the For t 2 protein translation in vivo , mice were sacrificed after removal of the vaccine patches for 24 and 48 hours, respectively. The vaccinated skin and spleen were removed for IHC with specific For t 2 polyclonal antibody. As shown in Figure S3, For t 2 proteins were detected in the targeted skin and the immune organ spleen at 24 hours and became more significant at 48 hours.