2.3∣Detection of For t 2 protein expression in the skin
Intradermal expression of the For t 2 proteins was evaluated. The For t 2 DNA vaccine was skin-patched for 1 hour then removed from the abdominal skin of the mouse. Twenty-four and 48 hours after being vaccinated, two mice were sacrificed, respectively. The skins from the vaccinated sites were collected and immersed in 10% formalin overnight at room temperature. Briefly, 5-μm sections were subjected to detection with rabbit anti-For t 2 (1200-fold dilution) and then incubated with peroxidase-conjugated goat anti-rabbit IgG and stained in a DAB solution using Bond automatic system (Leica, Newcastle, UK).