Figure 5. TMTM reduces plant response towards sulfur deficiency. Gene expression was analyzed after 2 days (A) and 7 days (B). Values were normalized to seedlings grown on low sulfate (LS) MGRL medium without TMTM (0 μg), and expressed as fold change. RNA for each treatment was extracted from total seedlings (combining root and shoot). Error bars represent SEs from 3 independent biological replicates, each with 8 seedlings. Statistical significance was calculated from dCq values, determined by Duncan’s multiple range test with p-value < 0.05, and indicated with lower-case alphabets.