2.11 Measurement of corrosion potential and corrosion current of
an Fe0 electrode
Electrochemical analyses were conducted at 25ºC in an electrochemical
cell (8 ml in capacity) equipped with three electrodes, as described by
Okamoto et al. (2014), with slight modifications. The working
electrode was an Fe0 foil with a surface area of
3.14 cm2, which was placed on the bottom of the
electrochemical cell, while the counter and reference electrodes were a
platinum wire and an Ag/AgCl/(saturated KCl) electrode, respectively.
Filter-sterilized corrosion-test medium was used as an electrolyte into
which a cell suspension was injected to a final optical density at 660
nm of 0.02 to start the measurement. The corrosion potential of the
working electrode was measured continuously, except that, every 8 h, the
corrosion potential of the working electrode was swept at ±25 mV versus
the corrosion potential for the measurement of the corrosion current of
the working electrode.