Material and Methods
Schizochytrium sp. micro algae species was used as the study material. Spray-dried samples were obtained from Marin Biotechnology Products and Food Industry (Aydın/Turkey).
In order to increase the lipid yield of this algae, 4 different cell disruption methods were tried; treatment with HCl in the reflux, osmotic shock, enzyme and ultrasonic homogenizer. After each cell disruption method, classical method of Bligh and Dyer (1959) and Soxhlet extraction methods of AOAC (2006) were applied for total crude lipid. Bligh and Dyer (1959) and AOAC (2006) Soxhlet methods applied alone were accepted as control groups. The groups were coded as follows; Bligh and Dyer and Soxhlet groups; control (BDC and SC), osmotic shock (BDOS and SOS), enzyme (BDE and SE), HCl (BDHCl and SHCl), ultrasonic homogenizer (BDUH and SUH); respectively. In each method, 2 g of dry algae samples were used. Each analysis was carried out in three parallel.