Soxhlet method
Samples were placed in cartridges and put into the VELP SCI SER 148-Italy brand automatic soxhlet mechanism and hexane (60 mL) was used for lipid seperation. Process of immersion for 90 minutes at 180 °C, washing for 120 minutes at 80 ˚C, and finally recovery for 8 minutes was carried out in the device (AOAC, 2006).