Antibacterial Test of Hydrogel Formulations
500 µL of 100µM[AgNPs-PVP]-18%[P407] was pipetted into a 5 mL
round bottom tube and stored at 37°C until the formulations became
solid-like gel. Next, the 500 µL of prewarmed bacterial broth was added
into the tube. The tube was subsequently incubated at 37 °C in a
humidified 5% CO2-containing atmosphere for 24 hours.
At the end of the incubation, 100 µL suspension was spread onto
appropriate agar plates following the spread plates technology and
incubated for 24 hours before colony forming units were counted.