Biocompatibility Evaluation of AgNPs, PVP, and AgNPs-PVP
PC-12 cell line (ATCC CRL-1721.1) was cultured with F-12K medium
supplemented (Corning, USA) with 2.5% fetal bovine serum (Gibco, USA),
15% horse serum (Gibco, USA) and 1% penicillin and streptomycin
(Gibco, USA); and maintained in a humidified 5%
CO2-containing balanced-air incubator at 37°C.
PC-12 cells used in the assays were seeded in each well in the 96-well
plates at the density of \(1\times 10^{4}\) cells per well overnight at
37 °C in a humidified 5% CO2-containing atmosphere.
After that, the media in each well was discarded. And cells were exposed
to the AgNPs-PVP at concentrations of 3.125 µM, 6.25 µM, 12.5 µM, 25 µM,
50 µM, and 100 µM for 24h and 48h dissolved in fresh medium. Viability
of PC-12 cells was evaluated using the CCK-8 kit for mammalian cells
(Dojindo Molecular Technologies, Japan). The absorbance at 450 nm was
measured after incubating cells with the CCK-8 assay for 1-2h. Empty
wells with CCK-8 assay only were used as blanks and were subtracted from
the final reading. Relative cell viability was calculated by normalizing
the absorbance readings by that of untreated cells . All assays were
carried out in quadruplicates.