Mapping
Paired-end pool-seq reads described previously (Keeling et al., 2013c)
were obtained from the NCBI SRA database (Fairview (FV) - SRR073440;
Kananaskis (KA) - SRR086167; Terrace (TR) -SRR073431; Whitecourt (WC) -
SRR073441; Cypress Hills (CH) - SRR086168; Valhalla (VA) - SRR086169;
Houston (HO) - SRR086170). Paired-end reads were trimmed using BBDuk
v37.25 (Bushnell, 2020) (trimq = 20, minlength = 10) prior to mapping.
Two datasets containing northern (HO, TR, WC, FV) and southern (VA, KA,
CH) Canadian populations were created and mapped to the final female
genome assembly using the BBMap v36.92 (Bushnell, 2020) plugin for
Geneious v10.1.3 (Biomatters, Auckland, New Zealand) with default
settings.