Hi-C library preparation and sequencing
Dovetail Genomics LLC prepared a Hi-C library in a manner similar to that described previously (Lieberman-Aiden et al., 2009). Briefly, chromatin from another individual female beetle (NCBI BioSample: SAMN14918916) was fixed in place with formaldehyde in the nucleus and then extracted fixed chromatin was digested with DpnII . The 5’ overhangs were filled in with biotinylated nucleotides and then the free blunt ends were ligated. After ligation, crosslinks were reversed, the DNA was purified from protein, and a library was prepared as above and sequenced on an Illumina HiSeq X platform to produce 514 million 2x150 bp paired-end reads (NCBI SRA: SRR11965415), which provided 37 483 x physical coverage of the genome (10-10 000 kb pairs).