Hi-C library preparation and sequencing
Dovetail Genomics LLC prepared a Hi-C library in a manner similar to
that described previously (Lieberman-Aiden et al., 2009). Briefly,
chromatin from another individual female beetle (NCBI BioSample:
SAMN14918916) was fixed in place with formaldehyde in the nucleus and
then extracted fixed chromatin was digested with DpnII . The 5’
overhangs were filled in with biotinylated nucleotides and then the free
blunt ends were ligated. After ligation, crosslinks were reversed, the
DNA was purified from protein, and a library was prepared as above and
sequenced on an Illumina HiSeq X platform to produce 514 million 2x150
bp paired-end reads (NCBI SRA: SRR11965415), which provided 37 483 x
physical coverage of the genome (10-10 000 kb pairs).