Analytical specificity
To detect A. baumannii and K. pneumonia in blood samples, we designed a specific primer-probe set for each strain. The specificity test showed that all 5 A. baumannii isolates and 33 K. pneumonia isolates from ATCC were detected by corresponding primer-probe set, whereas the 131 negative control isolates showed no response to the primer-probe sets (Table 4 ). These results suggest that the primer-probe sets are specific to A. baumanniiand K. pneumonia , respectively.