3.10 CCK-8 Assay
In order to evaluate the proliferation of HUVECs cultured onto A-SA-Gel and SA/Gel hydrogel scaffolds, CCK-8 assay test was performed on day 1, 3, and 5 after cell culture and the obtained data is shown in Figure 9G. It was demonstrated that, compared with day 1, the OD values at a wavelength of 450 nm increased by around 2 times in all groups after culturing for 3 days. The cell growth in all groups was seen to be sharply improved on day 5, and the OD values of the A-SA-Gel group increased by about 6 times than that of day 1. Moreover, the OD values of the A-SA-Gel group even more than the control and SA/Gel groups. These results indicated that the cells can maintain their proliferation ability when cultured with the fabricated A-SA-Gel hydrogel scaffolds. Due to their favorable characteristics, such as cell compatibility and non-toxicity, the 3D printed A-SA-Gel hydrogel scaffolding system may find application in various tissue or organ engineering.