Figure 6 . A: Maximum likelihood SNP-based phylogeny and genetic
structure of generalized and thick-lipped ecomorphs ofLabeobarbus from the different basins of Ethiopia (Genale,
Didessa, Gojeb, Sore, and Birbir Rivers). The tree inferred with
IQ-TREE2 using 15,820 variant SNPs with the TVM+F+ASC+R3 substitution
model. Heterozygous sites within each individual were encoded using
IUPAC notation. The thick-lipped individuals in the tree are depicted in
yellow color. Individuals are labeled by their voucher numbers.
Ultrafast bootstrap values above 70% are shown as numbers near the
corresponding nodes, while the black points in the nodes designate
95-100% support. The genetic cluster proportions inferred by
hierarchical STRUCTURE analysis are shown to the right of sample
numbers: 1 - analysis of all samples with 2,411 SNPs revealed best K=4;
2 - analysis of the Genale genetic cluster with 7,905 SNPs revealed no
further subdivision; 3 - analysis of the Didessa genetic cluster with
6,932 SNPs revealed subdivision on two clusters (best K=2) corresponding
to mouth phenotypes; 4 - analysis of the Gojeb genetic cluster with
4,471 SNPs revealed no further subdivision; 5 - analysis of the
Sore/Birbir (White Nile basin) genetic cluster with 2,020 SNPs revealed
subdivision on two clusters (best K=2) corresponding to the river
basins. B: Principal Component Analysis (PCA) plot with points
representing individuals and 95% confidence intervals colored by
genetic clusters inferred with STRUCTURE.