2.3.Statistical analyses:
Statistical analyses were performed with SPSS v.24. The chi-square test was used the determine correlations among the variables. Statsitically analyses of prognostic effects of pathological tumour stage, LNM, LVI, PNI, necrosis and EMT markers on OS were performed. Univariate OS were estimated using the Kaplan-Meier method and log-rank statistics. Multivariate Cox regression models addresses OS. Statsitical significance was set at p<0.05.