Western blot analysis
The samples of protein from sciatic nerves were loaded into 12%
SDS-PAGE gels, then transferred to PVDF membrane (Millipore Billerica,
MA, USA). The following antibodies were used: rabbit anti-NF-κB p65 and
anti-NF-κB p-p65 monoclonal antibody (Cell Signaling Technology,
Danvers, MA, USA, 1:1000); β-actin (Bioss, China, 1:2000) and the
corresponding horseradish peroxidase (HRP)-conjugated secondary
antibodies (Bioss, China, 1:1000). The blots were developed with
enhanced chemiluminescence kit (Amersham Imager 600, GE, USA).
Densitometric analysis of western blots were done using ImageJ software.