M2 macrophages treatment reduced the expression of M1 macrophages and reduced the activation of NF-κB in EAN
The data of flow cytometric displayed reduced expression of M1 macrophages (both CD40 and iNOS positive) compared to the control group (P﹤0.05), and elevated expression of M2 macrophages (either CD206 or Arg-1 positive) from spleen MNCs (P﹤0.05) (Figure 2c and d).
Western blotting results indicated that M2 macrophages treatment reduced the expression of NF-κB p-p65 in sciatic nerves of EAN mice on the peak phase compared to the control group (P﹤0.05) (Figure 2e).