Western blot analysis
The samples of protein from sciatic nerves were loaded into 12% SDS-PAGE gels, then transferred to PVDF membrane (Millipore Billerica, MA, USA). The following antibodies were used: rabbit anti-NF-κB p65 and anti-NF-κB p-p65 monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA, 1:1000); β-actin (Bioss, China, 1:2000) and the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Bioss, China, 1:1000). The blots were developed with enhanced chemiluminescence kit (Amersham Imager 600, GE, USA). Densitometric analysis of western blots were done using ImageJ software.