Vaccination regimen/dose/sera collection
Wild type BALB/cOlaHsd mice were vaccinated subcutaneously (s.c.) using
different regimens and doses. After comparing the efficacy of the
different regimens and doses, 100µg of the vaccine or a control
(CuMVTT-VLPs, do not display an epitope) was used in the
experiments showed in this study. 100µg (Prime on day 0) and 100µg for a
booster dose on day 14 or 28 were used. CuMVTT-RBM
candidate vaccine was diluted in 20 mM Tris, 2 mM EDTA in a final volume
of 100µl for final injection. Serum was collected on a weekly basis.
Three doses regimen of each 100µg were given at days 0, 28 and 42.