RBD mutations protein expression and purification
SARS-CoV-2 RBDwildtype, RBDK417N,
RBDE484K, RBDN501Y,
RBDK417N/E484K/N501Y,
RBDL452R/E484Q were cloned as a synthetic gene into
pTWIST-CMV-BetaGlobin-WPRE-Neo vector (Twist Biosciences, CA, USA) and
expressed in HEK293F cells through the Expi293 system (ThermoFisher
Scientific, MA, USA). Purification was performed by IMAC using a HiTrap
TALON crude column (Cytiva, Uppsala, Sweden).