3.1. Probes for CBH
In addition to the fifteen 16S rDNA probes available, seventeen
explorative probes targeting 18S rDNA were designed to recover a broad
range of eukaryotes, with a special emphasis on metazoans. Probe
positions within 16S and 18S rDNA genes are visualized in Appendix 2.
The coverage of the two sets of probes, determined in silico ,
reached 99.99% for 16S rDNA and 99.98% for 18S rDNA of the 369953 and
55145 targeted taxa present in the SILVA SSU NR99 (version 132; see
Appendix 3), indicating a high potential sensitivity of probes for the
recovery of prokaryotic and eukaryotic diversity.