3.1. Probes for CBH
In addition to the fifteen 16S rDNA probes available, seventeen explorative probes targeting 18S rDNA were designed to recover a broad range of eukaryotes, with a special emphasis on metazoans. Probe positions within 16S and 18S rDNA genes are visualized in Appendix 2. The coverage of the two sets of probes, determined in silico , reached 99.99% for 16S rDNA and 99.98% for 18S rDNA of the 369953 and 55145 targeted taxa present in the SILVA SSU NR99 (version 132; see Appendix 3), indicating a high potential sensitivity of probes for the recovery of prokaryotic and eukaryotic diversity.