3.4. Statistical analyses comparing CBH with
metabarcoding
The ten samples chosen for this comparative study were collected at five
different sites, including two different ecosystems, seamounts (in the
Atlantic Ocean and the Mediterranean) and hydrothermal vents (inactive
site, characteristic of deep bathyal sediment systems), to test
different biological communities. Based on multidimensional analysis
from Bray Curtis (based on the number of sequences) distances of
CBH-short and MTB methods for prokaryote communities, the methods
clearly appeared to be the dominant driver of differentiation (Appendix
6A). Mantel test showed that all three analyses were significantly
different from each other (p<0.01). In contrast, for 18S
detection, clusters were segregated per location of origin rather than
method (Appendix 6B). Mantel test showed no significant difference
(p=0.02), suggesting less technical bias in the community description of
eukaryotes.