Figure 3. DENV2-induced NOD2-MAVS co-localization in THP-1 macrophage-like cells. (A-C) Untreated (UT), DENV2-infected and PIC-transfected cells can be appreciated at 12 hpi. To evaluate NOD2-MAVS co-localization, the cells were fixed, permeabilized and immunostained with an anti-MAVS antibody ( in red, D-F) and an anti-NOD2 antibody ( in green,G-I) . To the far right, the images of MAVS (red) and NOD2 (green) are merged, with a nuclear contrast (blue). Individual cells displayed in the square were selected for observation in greater detail. A digital magnification of 630x is illustrated on the left and 2100x on the right side of each column. Each condition was repeated three times. Overlap coefficient provided by JACoP program is shown for DENV treated (0.859) and L18-MDP positive control (0.890) after analyzing different fields.