Figure 2. DENV2-induced NOD2-RIP2 co-localization is
demonstrated in THP-1 macrophage-like cells. (A-C) Untreated
(UT), DENV2-infected and L18-MDP-transfected cells are illustrated at 6
hpi. To evaluate NOD2-RIP2 co-localization, the cells were fixed,
permeabilized and immunostained with an anti-RIP2 antibody ( in
red, D-F) and an anti-NOD2 antibody ( in green,G-I) . To the far right, the images of RIP2 (red) and NOD2
(green) are merged, with a nuclear contrast (blue). Individual cells
portrayed in the square were selected for examination in greater detail.
A digital magnification of 630x is shown on the left and of 2100x on the
right side of each column. Each condition was repeated three times.
Overlap coefficient provided by JACoP program is shown for DENV treated
(0.776) and L18-MDP positive control (0.869) after analyzing different
fields.