Introduction: We aimed to measure the dynamic thiol-disulfide
balance and thioredoxin reductase (TrxR) enzyme levels in patients with
chronic kidney disease (CKD).
Methods: Thirty hemodialysis (HD), 30 CKD patients (stage3-5)
and 30 controls were included in the study. The dynamic thiol-disulfide
balance was determined by the colorimetric method developed by Erel et
al. TrxR levels were determined by ELISA.
Results: Native and total thiol levels of CKD and HD patients
were significantly lower than that of the control group (p=0.001for
both). However, disulfide levels were significantly higher in the HD
group (p=0.001), but there was no significant difference between control
and CKD groups(p=0.547). A notable negative correlation was found
between the native and total thiol levels and IMA(r=-0.628;-0.631),BUN
(r=-0.747;-0.747),and creatinine(r=-0.732;-0.721). There was a
significant positive correlation between GFR and the thiol levels
(r=0.835;0.824). TrxR levels were significantly higher in the patient
groups compared to the controls (p=0.001).CRP levels of the patient
groups were significantly higher compared to the controls (p=0.001).
Conclusion: We have demonstrated that measurement of dynamic
thiol-disulfide levels by using colorimetric method can contribute to
the diagnosis and follow-up of the disease as a marker, because, it is
easily applicable in routine clinical biochemistry laboratories and
related with disease severity in CKD patients. Also, we showed that
volume correction due to dialysis process should be consider in studies
dealing with plasma thiol values and the final results should be given
after the correction process.
Keywords: Chronic kidney disease, hemodialysis, oxidative
stress, thiol- disulphide homeostasis, thioredoxin reductase
What is known
- CKD is a syndrome characterized by the progressive and irreversible
loss of nephrons due to various diseases.
- Oxidative balance is disrupted due to overproduction of free radicals
and insufficient antioxidant system in HD patients.
- The main target of free radicals is thiol groups in the
sulfur-containing amino acids of proteins.
What’s new
Volume correction, which may be caused by this dialysis process,
should be taken into account in studies dealing with plasma thiol
values and the final results should be given after the correction
process.
Measurement of dynamic thiol-disulfide levels by using colorimetric
method can contribute to the diagnosis and follow-up of the disease as
a marker.