Assessment of grip strength
Skeletal muscular strength in mice was quantified with the grip strength
test. At the end of the experiment, the forelimb grip strength of each
of the four limbs was measured with a Digital Grip Strength Metre
(Nanjing Biomedical Research Institute of Nanjing University). Mice were
held by the tail and allowed to grasp a grid with their forepaws or with
all four paws. The mice were gently pulled by the tail until they
released their grip. Five measurements at 3 min intervals were recorded,
and the average value for each mouse was determined.