Figure 1. Enhanced HSP90-STAT3 interaction induced prolonged STAT3 activation in the skeletal muscle from cancer cachectic patients and C26 tumor-bearing cachectic mice.
The expression levels of Atrogin-1, MuRF-1, MHC, HSP90, Myostatin, and phosphorylated STAT3 in the skeletal muscle from cancer cachectic patients were detected. (a) the expressions of Atrogin-1 and MuRF-1 were assessed by qRT-PCR (b) the expressions of Atrogin-1, MuRF-1, MHC, HSP90, Myostatin, and phosphorylated STAT3 were detected by Western blot assay. (c) Measurement of the HSP90-STAT3 interaction in cachectic muscle cancer cachectic patients by immunoprecipitation. (d, e) The expression levels of Atrogin-1, MuRF-1, MHC, HSP90, Myostatin, and phosphorylated STAT3 in the skeletal muscle from C26 tumor-bearing cachectic mice were detected by (d. qRT-PCR for atrogin-1 and MuRF-1 and e. Western blot for Atrogin-1, MuRF-1, MHC, HSP90, Myostatin, and phosphorylated STAT3). (f) The lysates of C26 or control mice muscle were immunoprecipitated with the STAT3 antibody, the HSP90 antibody, or control rabbit IgG. The immunopellets were detected by immunoblot analysis with the anti-HSP90 antibody and the anti-STAT3 antibody