Figure 1. Enhanced HSP90-STAT3 interaction induced prolonged
STAT3 activation in the skeletal muscle from cancer cachectic patients
and C26 tumor-bearing cachectic mice.
The expression levels of Atrogin-1, MuRF-1, MHC, HSP90, Myostatin, and
phosphorylated STAT3 in the skeletal muscle from cancer cachectic
patients were detected. (a) the expressions of Atrogin-1 and
MuRF-1 were assessed by qRT-PCR (b) the expressions of
Atrogin-1, MuRF-1, MHC, HSP90, Myostatin, and phosphorylated STAT3 were
detected by Western blot assay. (c) Measurement of the
HSP90-STAT3 interaction in cachectic muscle cancer cachectic patients by
immunoprecipitation. (d, e) The expression levels of Atrogin-1,
MuRF-1, MHC, HSP90, Myostatin, and phosphorylated STAT3 in the skeletal
muscle from C26 tumor-bearing cachectic mice were detected by
(d. qRT-PCR for atrogin-1 and MuRF-1 and e. Western
blot for Atrogin-1, MuRF-1, MHC, HSP90, Myostatin, and phosphorylated
STAT3). (f) The lysates of C26 or control mice muscle were
immunoprecipitated with the STAT3 antibody, the HSP90 antibody, or
control rabbit IgG. The immunopellets were detected by immunoblot
analysis with the anti-HSP90 antibody and the anti-STAT3 antibody