2.1 Sample collection and genome sequencing
Fenneropenaeus chinensis shrimp were obtained from the
conservation base of Haifeng Aquaculture Co., Ltd. (Weifang, Shandong
Province, China). Muscle of a 7-months-old male shrimp was collected and
frozen in liquid nitrogen immediately. Total genomic DNA was extracted
and sequenced for genome survey and genome construction. Genome
construction contains PacBio sequencing (Eid et al., 2009) and Hi-C
assembly (Lieberman-Aiden et al., 2009). DNA sample used for genome
survey and Hi-C assembly was sequenced by Illumina HiSeq platform
(Illumina, San Diego, USA), and used for PacBio sequencing was sheared
to 20 Kb and sequenced by PacBio Sequel platform (Pacific Biosciences,
Menlo Park, USA).