4.1 Quality of this assembled genome improved
Compared to the published genome of F. chinensis (Yuan et al.,
2021), the quality of our assembled genome improved significantly (Table
S20). Primarily, the contig N50 increased from 59.00 Kb to 472.84 Kb,
approximately eight times, and scaffold N50 increased from 28.92 Mb to
36.87 Mb. Secondly, the assembled complete ortholog proportion enhanced
from 83.58% to 94.00% according to the BUSCO assessment, and the
genome coverage enhanced from 82.46% to 96.15% according to the
Illumina short read mapping test. In short, our assembled genome
improved in both fragment length and completeness.
Initially, we assembled the contigs to 44 pseudochromosomes, but the
heatmap revealed a low correlation between the contigs on the last
pseudochromosome (Figure S5). Therefore, only 43 pseudochromosomes were
assembled in the final version of the F. chinensis genome. We
speculate that there are only 43 pairs of chromosomes in the somatic
cell of F. chinensis , same as
that in Marsupenaeus japonicus (Yuan et al., 2017).