Figure Legends.
Figure 1. Immunohistochemical demonstration of localization of
CGRP, CT, Amylin and Adrenomedullin in the trigeminal ganglion.A. CGRP is expressed in a granular pattern in many neurons,
mainly in small- to medium-sized neurons (arrow heads). The cellular
CGRP is expressed in vesicles (insert). In addition, pearl-like CGRP
immunoreactivity was detected in fibers that are of the C-type of
sensory unmyelinated nerves (long arrows). The myelinated fibers do not
contain CGRP. Short arrow points at a large negative neuron. B .
CT immunoreactivity displayed a similar pattern as for CGRP; granular
staining of small- to medium-sized neurons (arrow heads, insert) and
pearl-like staining of fibers. Also, SGCs were CT immunoreactive
(asterisks). Arrows point at a large negative neuron. C . AMY
was exclusively expressed in the neurons, mainly small to medium sized
(arrow heads). In some of the cells, the expression was granular, but in
others, a general cytoplasmic immunoreactivity. Arrows point at a large
negative neuron. Insert shows three different cells: one negative and
two amylin immunoreactive. D . AM was expressed in the glial
cells, both the SGCs (arrow heads) and cells enveloping the neuronal
processes (arrows), probably myelinating cells.
Figure 2. Schematic illustration of the differential
distribution of the CGRP family of peptides and receptors. The CGRP
receptor (CLR/RAMP1) is mainly expressed in the large neurons and SGCs,
the Adrenomedullin2 receptor (CLR/RAMP3) in some SGCs,
the Amylin1 receptor (CTR/RAMP1) in large neurons and
SGCs, and the Amylin3 receptor (CTR/RAMP3) in some SGCs
Modified from Edvinsson, Grell & Warfvinge, 2020.