3.1 Cell viability analysis
In order to select the best concentration of Twist1 inhibitor that did
not affect the viability of primary HUVECs, cells were treated with
different concentrations of harmine (0, 1, 3, 6, 16, 32, 64, 128μmol/L)
for 48h successively. Then we used the CCK-8 method to determine the
effects of each concentration on cel l survival rate. Figure 1 shows
that as the concentration of harmine increased, the cell viability
became lower and lower. Compared with 0μmol/L, 1μmol/L and 3μmol/L of
harmine had no effect on HUVEC viability (p>0.05). When the
concentration of harmine was 6μmol/L and above, significant cell death
was induced (p<0.001). When the concentration of harmine was
16μmol/L, the cell viability was only about 50%. Moreover, the 10%
inhibitory concentration (IC10) of the harmine was about
2.7 by spss software. On the basis of this data, a concentration of
3μmol/L was selected as the optimal concentration for harmine to use in
subsequent experiments.