3.2 Changes in the protein expression of Ang1, Ang2, Twist1 and Tie2 in primary HUVECs under hyperoxia
Previously, we used western blot to detect the expression of Ang1, Ang2, Twist1 and Tie2 proteins in the lung tissue of hyperoxia neonatal rats20, but did not further verify the changes of these proteins by performing in vitro experiment. As shown in Figure 2, compared with the control group, the protein expression of Twist1 (p <0.05) (Figure 2A, 2B), Tie2 (p <0.01) (Figure 2A,2C) and Ang1 (p <0.01) (Figure 2A,2D) were significantly reduced in the hyperoxia group. In contrast, Ang2 protein expression in the hyperoxia group was significantly higher than that in the control group (p <0.01) (Figure 2A, 2E). The results of this in vitro experiment were consistent with those derived from the in vivo experiment.