3.1 Cell viability analysis
In order to select the best concentration of Twist1 inhibitor that did not affect the viability of primary HUVECs, cells were treated with different concentrations of harmine (0, 1, 3, 6, 16, 32, 64, 128μmol/L) for 48h successively. Then we used the CCK-8 method to determine the effects of each concentration on cel l survival rate. Figure 1 shows that as the concentration of harmine increased, the cell viability became lower and lower. Compared with 0μmol/L, 1μmol/L and 3μmol/L of harmine had no effect on HUVEC viability (p>0.05). When the concentration of harmine was 6μmol/L and above, significant cell death was induced (p<0.001). When the concentration of harmine was 16μmol/L, the cell viability was only about 50%. Moreover, the 10% inhibitory concentration (IC10) of the harmine was about 2.7 by spss software. On the basis of this data, a concentration of 3μmol/L was selected as the optimal concentration for harmine to use in subsequent experiments.