2.1 Cell culture
Primary HUVECs were purchased from American Sciencecell company and
cultured in endothelial cell medium (ECM) in a 37℃ 5%
CO2 saturated humidity incubator. The flasks or dishes
used to culture primary HUVECs were coated with fibronectin ( 2
μg/cm2) overnight at 37℃. When the cell fusion rate
reached 90%-95%, cells from the logarithmic growth phase were digested
with trypsin cell digestive solution at a concentration of 0.05% and
then sub-cultured. HUVECs were taken from generations 3-6 and used in
subsequent experiments.