Suitable conditions for high-value compounds hyperaccumulation are the most challenging approach in Arthrospira platensis. In the current study, the co-production of specialty pigments and high-value metabolites was investigated with a varying concentration of well-known and candidate metabolic stressors in Arthrospira platensis. Supplementing Arthrospira platensis with a minimum of 0.50% of Monosodium glutamate (MSG) and a maximum of 2% of aspartate (ASP) increased Phycocyanin productivity by 83.36 and 64.77 percent, respectively. However, a maximum of 1.50% of MSG and a minimum of 0.50% ASP increased carotenoid content by 188.80 and 68.97 percent, respectively. These observations, coupled with those showing supplemented ASP stimulate unsaturated fatty acid accumulation, possibly through enhanced de novo biosynthesis, and essential amino acid biosynthesis to a greater extent in Arthrospira platensis. A network correlation analysis also indicated the distinct metabolite abundance and the corresponding associated metabolic networks linked to pigment production in Arthrospira platensis.