The fishing cat (Prionailurus viverrinus) is a medium-sized wetland specialist threatened felid with limited knowledge base on behavior and ecology. We carried out comprehensive analysis of its diet within a human-dominated landscape in southern Nepal, analyzing scat samples through microscopic analysis and DNA metabarcoding. The microscopic analysis revealed that mammals, primarily rodents, constituted the largest proportion of fishing cat diet (61.73% frequency of occurrence (FO)) followed by birds (43.21% FO) fish (24.69% FO) and reptiles (19.75% FO). However, DNA metabarcoding documented fish as the main diet (70% FO), followed by mammals (63.33% FO) and birds (30% FO). Our findings demonstrate that, fish alone is not a major food source of fishing cats indicate their role to regulate rodents and birds that damages the farm crops. The trophic niche breadth (0.68) shows the fishing cats a diet generalist. We recommend launching awareness programs and programs promoting integrated fish cum paddy farming among fish farmers within the fishing cat range, benefiting both farmers and fishing cat populations. Additionally, targeted conservation measures such as controlling physical and chemical pollution in agricultural and wetland sites are essential to mitigate threats to fishing cat populations. Prioritizing wetland health should be a central strategy in conservation efforts, which will benefit not only fishing cat, but also many co-distributed species.

Mammals have experienced a massive decline in their populations and geographic ranges worldwide. The sloth bear, Melursus ursinus (Shaw, 1791), is one of many species facing conservation threats. Despite being endangered in Nepal, decades of inattention to the situation have hindered their conservation and management. We assessed the distribution and patterns of habitat use by sloth bears in Chitwan National Park (CNP), Nepal. We conducted sign surveys from March to June, 2020, in 4 × 4 km grids (n = 45). We collected detection/non-detection data along a 4-km trail that was divided into 20 continuous segments of 200 m each. We obtained environmental, ecological, and anthropogenic covariates to understand determinants of sloth bear habitat occupancy. The data were analyzed using the single-species single-season occupancy method, with a spatially correlated detection. Using repeated observations, these models accounted for the imperfect detectability of the species to provide robust estimates of habitat occupancy. The model-averaged occupancy estimate for the sloth bear was 69% and the detection probability was 0.25. The probability of habitat occupancy by sloth bears increased with the presence of termites and fruits and in rugged, dry, open, undisturbed habitats. Our results indicate that the sloth bear is elusive, functionally unique, and widespread in CNP. Future conservation interventions and action plans aimed at sloth bear management must adequately consider their habitat requirements.

The fishing cat Prionailurus viverrinus is a wetland specialist endemic to South and Southeast Asia. Nepal represents the northern limit of its biogeographic range, but comprehensive information on fishing cat distribution in Nepal is lacking. We compiled fishing cat occurrence records (n=154) from Nepal, available in published literature and unpublished data (2009 – 2020), to assess their distribution. Bioclimatic and environmental variables associated with their occurrence were used to predict the potential fishing cat range using MaxEnt modeling. Fishing cat distribution was influenced by elevation, precipitation of the warmest quarter (18_bio), precipitation of the driest month (14_bio) and land cover. Wetlands and forest cover were the important predictors of fishing cat distribution. The model predicted an area of 4.4% (6,679 km2) of Nepal as potential habitat for the fishing cat. About two third of the predicted potentially suitable habitat lies outside protected areas, however a large part of the highly suitable habitat (67%) falls within protected areas. The predicted habitat map serves as a reference for future investigation into fishing cat distribution as well as formulating and implementing effective conservation programs for fishing cats in Nepal. Fishing cat conservation initiatives should include habitats both inside and outside the protected areas to ensure long-term survival. We recommend conservation of wetland sites, surveys of fishing cats in the identified potential habitats, and study of their genetic connectivity and population status.

Indiscriminate fire is rampant throughout subtropical South and Southeast Asian grasslands. However, very little is known about the role of fire and pyric herbivory on the functioning of highly productive subtropical monsoon grasslands lying within Cwa-climatic region. We collected grass samples from 60 m x 60 m plots and determined vegetation physical and chemical properties at regular 30-day intervals from April to July 2020, starting from 30 days after fire to assess post-fire regrowth forage quality. We counted pellet groups for the same four months from 2 m x 2 m quadrats that were permanently marked with pegs along the diagonal of each 60 m x 60 m plot to estimate grazing intensity to the progression of post-fire regrowth. We observed strong and significant reductions in crude protein (mean value 9.1 to 4.1 [55% decrease]) and phosphorus (mean value 0.2 to 0.11 [45% decrease]) in forage collected during different time intervals i.e., from 30 days to 120 days after fire. Mesofaunal deer utilised the burned areas extensively for a short period, i.e., up to two months after fire when the burned areas contained short grasses with a higher level of crude protein and phosphorus. Grazing intensity of chital (Axis axis) to post-fire regrowth differed significantly over time since fire, with higher intensity of use at 30 days after fire. Grazing intensity of swamp deer (Rucervus duvaucelii) did not differ significantly until 90 days after fire, however, decreased significantly after 90 days since fire. Large-scale indiscriminate single event fires thus may not fulfil nutritional requirements of all species in mesofaunal deer community in these subtropical monsoon grasslands. We recommend for a spatio-temporal manipulation of fire to reinforce grazing feedback and to yield for the longest possible period a reasonably good food supply for the conservation of mesofaunal deer.

Conservation of large carnivores like leopards requires large and interconnected habitats. Despite the wide geographic range of the leopard globally, only 17% of their habitat is within protected areas. In Nepal, leopards are distributed widely across the country but their status is not adequately studied which compromised the necessary conservation attention for the species. This study carried out sign-based occupancy survey across the Chure (the Himalayan foothills) range (~19,000 km2) to understand the habitat occupancy of leopards along with the covariates affecting their presence. The model-averaged leopard occupancy in the Chure range was 0.5732 (0.0082 SD) with a detection probability of 0.2554 (0.1142 SE). The top model included wild boar, ruggedness, presence of livestock and human population density as covariates. The β coefficient estimate from the model indicated the wild boar was the primary covariate contributing positively to the leopard occupancy followed by the presence of livestock, ruggedness and human population density. The detection probability of leopard was higher outside the protected areas, less in the densely vegetated areas, and higher in the area where there is a presence of livestock. Enhanced law enforcement and mass awareness activities are necessary to reduce poaching/killing of wild ungulates and leopard in the Chure range and to increase leopard occupancy. In addition, maintaining a sufficient natural prey base can contribute to minimize the livestock depredation and hence, decrease the human-leopard conflict in the Chure range.

Attacks on humans by Asian elephant (Elephas maximus) is an extreme form of Human-elephant conflict. It is a serious issue in southern lowland Nepal where elephants kill more humans than any other wildlife. Detailed understanding of elephant attacks on humans in Nepal is still lacking which affected in devising appropriate strategies and actions for human elephant conflict mitigation. This study documented spatio-temporal pattern of elephant attacks on humans, factors associated with the attacks and human/elephant behaviour contributing to deaths of victims when attacked. We compiled all the documented incidences of elephant attacks on humans in Nepal for last 20 years across Terai and Siwalik region of Nepal. We also visited and interviewed 412 victim families (274 fatalities and 138 injuries) on elephant attacks. Majority of the victims were males (87.86%) and had low level of education. One fourth of the elephant attacks occurred while chasing the elephants. Solitary bulls or group of sub-adult males were involved in most of the attack. We found higher number of attacks outside the protected area. People who were drunk and chasing elephants using fire-crackers were more vulnerable to the fatalities. In contrast, chasing elephants using fire was negatively associated to the fatalities. Elephant attacks were concentrated in proximity of forests primarily affecting the socio-economically marginalized communities. Integrated settlement, safe housing for marginalized community and community grain house in the settlement should be promoted to reduce the confrontation between elephants and humans. Conservation of elephant should be carried out in entire landscape, extending beyond the boundary of protected areas to reduce threats of elephant extinction.