Chloroquine, hydroxychloroquine and azithromycin block hERG at
physiological temperatures
The potency of each compound for hERG block was determined using a
Syncropatch PE384 automated patch clamp system. Typical current traces
and timecourse of block for ~IC50 doses
of chloroquine (1 µM), hydroxychloroquine (3 µM) and azithromycin (100
µM) are shown in Figures 1A-C. The potency of hERG inhibition
(IC50 ± SEM) was 1.47±0.07 µM, 3.78±0.18 µM, and
280.1±14.7 µM for chloroquine, hydroxychloroquine and azithromycin
respectively (Figure 1D).