Viral Stocks
The SARS-CoV-2 strain PMVL-1 (GISAID Number EPI_ISL_421275) was propagated and titrated in Vero E6 cells (ATCC®Number: CRL-1586TM) to determine TCID50/mL (50% tissue culture infectivity dose). This virus was used for experiments in vitro . The SARS-CoV-2 strain B was grown in Vero C1008 cells (ATCC® CRL-1586™) and used for in vivo experiments. SARS-CoV-2 strain B was titrated on Vero C1008 cells by plaque assay to determine the number of plaque forming units (PFU) as described(31). Viral stocks were used at 1.0 x 105 TCID50/mL and 1.0x106 PFU/mL for strain PMVL-1 and strain B of SARS-CoV-2, respectively.