Statistical analysis
The Shapiro-Wilk test for normality was performed. Data were presented
as means + s.e.m for parametric analyses and as medians + s.d. for
nonparametric analyses, as indicated in the figure legends. Statistical
analyses were performed using Prism 8 (GraphPad Software). For normally
distributed data we performed ordinary one-way ANOVA or one-way repeated
measures ANOVA with Tukey’s or Dunnetts’s post hoc tests, respectively,
for multiple comparisons to determine differences between groups, or if
not normally distributed the Kruskal-Wallis test followed by post hoc
testing (if the Kruskal-Wallis was significant) using un-paired
Mann-Whitney U tests was performed, as mentioned in the respective
figure legends. Data were accepted as significantly different when
P<0.05.