Transfection of HEp-2 cells with pGL-3/KK-46 complexes
The HEp-2 cells were propagated as described above. Cells were transfected with the pGL3 Luciferase Reporter Vector (Promega) (0.5 μg/well) in complexes with peptide dendrimer KK-46. In these experiments several weight ratios of KK-46 and vector (5:1,12.5:1, 20:1, 25:1, 50:1, 100:1 µg KK-46:µg pGL3) were used to transfect the cells. Commercial Lipofectamine 3000 was used as positive control. The transfection protocol and assays were performed as described above.